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JCM 841 . PROPIONISPORA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J841 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.198 1.458
NH4Cl 0.25 g 0.248 4.637
NaCl 1.00 g 0.992 16.976
MgCl2 x 6 H2O 0.40 g 0.397 1.952
CaCl2 x 2 H2O 0.15 g 0.149 1.012
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Resazurin 0.50 mg 5.0e-4 0.002
Yeast extract
(BD-Difco) 		1.00 g 0.992 -
Distilled water 900.00 ml - -
1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
Fructose
(10%) 		50.00 ml - -
NaHCO3
(*, 5%) 		50.00 ml - -
Na2S x 9 H2O
(5%) 		6.00 ml - -
2 Adjust pH to 7.0 - 7.2, if necessary.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.992 16.976
Yeast extract 0.992 -
MgCl2 x 6 H2O 0.397 1.952
NH4Cl 0.248 4.637
KH2PO4 0.198 1.458
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain