Name: LIND BHT MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J843 #

Equipment needed: Anaerobic workbench Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.30	g	0.297	2.185
K₂HPO₄	0.30	g	0.297	1.707
NH₄Cl	1.00	g	0.991	18.528
NaCl	20.00	g	19.822	339.179
KCl	0.10	g	0.099	1.329
CaCl₂ x 2 H₂O	0.10	g	0.099	0.674
Trace element solution	1.00	ml	-	-
Yeast extract	2.00	g	1.982	-
Na₂S₂O₃ x 5 H₂O	4.96	g	4.916	19.807
L-Cysteine HCl x H₂O	0.50	g	0.496	-
Resazurin	1.00	mg	9.9e-4	0.004
Distilled water	925.00	ml	-	-
1 Mix components thoroughly and autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO₃ (*, 8%)	25.00	ml	-	-
MgCl₂ x 6 H₂O (10%)	30.00	ml	-	-
Sodium acetate (1.0 M)	20.00	ml	-	-
2 Then, aseptically distribute the medium into culture vessels under a stream of H₂-CO₂ (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N₂ atmosphere):
Na₂S x 9 H₂O (5%)	8.00	ml	-	-
3 Pressurize inoculated culture vessels to 200 kPa H₂-CO₂ (4:1, v/v).

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	29.10.21
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	n.d.
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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