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JCM 843 . LIND BHT MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.30 g 0.297 2.185
K2HPO4 0.30 g 0.297 1.707
NH4Cl 1.00 g 0.991 18.528
NaCl 20.00 g 19.822 339.179
KCl 0.10 g 0.099 1.329
CaCl2 x 2 H2O 0.10 g 0.099 0.674
Trace element solution 1.00 ml - -
Yeast extract 2.00 g 1.982 -
Na2S2O3 x 5 H2O 4.96 g 4.916 19.807
L-Cysteine HCl x H2O 0.50 g 0.496 -
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 925.00 ml - -
1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%)
25.00 ml - -
MgCl2 x 6 H2O
(10%)
30.00 ml - -
Sodium acetate
(1.0 M)
20.00 ml - -
2 Then, aseptically distribute the medium into culture vessels under a stream of H2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere):
Na2S x 9 H2O
(5%)
8.00 ml - -
3 Pressurize inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.822 339.179
Na2S2O3 x 5 H2O 4.916 19.807
Yeast extract 1.982 -
NH4Cl 0.991 18.528
L-Cysteine HCl x H2O 0.496 -
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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