Name: RSULFAC1 MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J844 #

Equipment needed: Filter Autoclave Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.3	g	0.282	2.074
K₂HPO₄	0.3	g	0.282	1.62
NH₄Cl	1.0	g	0.941	17.587
NaCl	5.0	g	4.704	80.487
KCl	0.1	g	0.094	1.262
CaCl₂ x 2 H₂O	0.1	g	0.094	0.64
MgCl₂ x 6 H₂O	0.5	g	0.47	2.314
Trace element solution	10.0	ml	-	-
Yeast extract	1.0	g	0.941	-
Peptone	2.0	g	1.881	-
L-Cysteine HCl x H₂O	0.5	g	0.47	-
Sulfur (powder)	10.0	g	9.407	293.338
Resazurin	1.0	mg	9.4e-4	0.004
Distilled water	1000.0	ml	-	-
1 Mix components except sulfur thoroughly and autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized):
NaHCO₃ (*, 8%)	25.0	ml	-	-
Sodium fumarate (1.0 M)	20.0	ml	-	-
2 Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium and sulfur into culture vessels under a stream of N₂-CO₂ (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N₂ atmosphere):
Na₂S x 9 H₂O (5%)	8.0	ml	-	-

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	29.10.21
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	n.d.
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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