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JCM 850 . THERMOSYNTROPHA L-60 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

760
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.54 g 0.711 5.221
Na2HPO4 1.33 g 1.75 12.328
Yeast extract
(BD-Difco) 		3.00 g 3.947 -
Tryptone
(BD-Difco) 		0.50 g 0.658 9.256
Bacto peptone
(BD-Difco) 		1.50 g 1.974 -
Resazurin 1.00 mg 0.001 0.006
Distilled water 760.00 ml - -

Solution B #

102
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 2.941 54.986
NaCl 0.30 g 2.941 50.328
CaCl2 x 2 H2O 0.01 g 0.098 0.667
MgCl2 x 6 H2O 0.10 g 0.98 4.822
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Distilled water 100.00 ml - -

Solution C #

140
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Crotonic acid 1.72 g 12.286 142.708
Distilled water 100.00 ml - -
1 Autoclave Solutions A, B and C separately. After cooling, aseptically and anaerobically combine the three solutions under a stream of N2-CO2 (80:20, v/v), then add the following solutions (filter-sterilized):
Trace vitamins 10.00 ml - -
NaHCO3
(8%) 		30.00 ml - -
2 Adjust pH to 7.0.
3 Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Prior to inoculation, reduce the medium with 0.025% L-cysteine x HCl x H2O and 0.025% Na2S x 9H2O (use 5% solutions autoclaved and stored under a N2 atmosphere).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain