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JCM 852 . SPIROCHAETA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J852 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.1 g 0.1 0.732
MgCl2 x 6 H2O 4.0 g 3.984 19.597
CaCl2 x 2 H2O 1.0 g 0.996 6.775
NH4Cl 0.5 g 0.498 9.31
NaCl 25.0 g 24.9 426.085
Yeast extract 1.0 g 0.996 -
Sucrose 1.7 g 1.693 4.947
Maltose 1.7 g 1.693 4.947
Vitamin solution 2.0 ml - -
Trace mineral solution 1.0 ml - -
Se/W solution 1.0 ml - -
NaHCO3 2.0 g 1.992 23.713
Resazurin 1.0 mg 1.0e-3 0.004
L-Cysteine HCl x H2O 0.3 g 0.299 -
Na2S x 9 H2O 0.3 g 0.299 1.244
Distilled water 1000.0 ml - -

Trace mineral solution #

1000
Solution occurs in 8 other media (first defined in Medium J852).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 1.990 g 1.99 10.01
MnCl2 x 4 H2O 0.198 g 0.198 -
CoCl2 x 6 H2O 0.130 g 0.13 1.001
ZnCl2 0.136 g 0.136 0.998
CuCl2 x 2 H2O 1.700 mg 0.002 0.01
H3BO3 6.200 mg 0.006 0.1
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.013 g 0.013 0.1
AlCl3 0.013 g 0.013 0.097
Distilled water 1000.000 ml - -

Se/W solution #

1000
Solution occurs in 3 other media (first defined in Medium J852).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 1.7 mg 0.002 0.006
Na2WO4 x 2 H2O 3.3 mg 0.003 0.01
Distilled water 1000.0 ml - -

Vitamin solution #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.9 mg 0.005 0.02
Folic acid 8.8 mg 0.009 0.02
Pyridoxine hydrochloride 4.1 mg 0.004 -
Thiamine HCl 6.7 mg 0.007 -
Riboflavin 7.5 mg 0.008 0.02
Nicotinamide 2.5 mg 0.003 0.02
DL-Calcium pantothenate 9.5 mg 0.01 0.02
Vitamin B12 27.1 mg 0.027 0.02
p-Aminobenzoic acid 2.7 mg 0.003 0.02
Lipoic acid 4.1 mg 0.004 0.02
Distilled water 1000.0 ml - -
1 For vial cultures, dissolve all compounds except Na2S x 9H2O and L-cysteine x HCl x H2O, then, adjust pH to 7.5. Dispense 19 ml of medium into 50-ml vials, purge with N2-CO2 (80:20) gas and fix with a stopper. After autoclaving, add filter-sterilized Na2S x 9H2O and L-cysteine x HCl x H2O solution to each vial as reducing agents.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.9 426.085
MgCl2 x 6 H2O 3.984 19.597
NaHCO3 1.992 23.713
Sucrose 1.693 4.947
Maltose 1.693 4.947
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
nutrition chemoheterotroph 1 strain
oxygen anaerobe 2 strains
temperature psychrophilic 2 strains