Name: DESULFOSPOROSINUS BSREI1 MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J865 #

Equipment needed: Autoclave Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
(NH₄)₂SO₄	0.450	g	0.446	3.375
KH₂PO₄	0.050	g	0.05	0.364
MgSO₄ x 7 H₂O	0.500	g	0.496	2.011
KCl	0.050	g	0.05	0.665
Ca(NO₃)₂ x 4 H₂O	0.014	g	0.014	0.059
Na₂SO₄	3.000	g	2.973	20.932
Trace element solution	1.000	ml	-	-
Yeast extract	0.500	g	0.496	-
L-Cysteine HCl x H₂O	0.500	g	0.496	-
Resazurin	1.000	mg	9.9e-4	0.004
Distilled water	910.000	ml	-	-
1 Mix components thoroughly, distribute the medium into culture vessels under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 910 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO₃ (*, 5%)	60.000	ml	-	-
Fructose (1 M)	20.000	ml	-	-
Trace vitamins (see Medium No. 197) (*)	10.000	ml	-	-
Na₂S x 9 H₂O (5%)	8.000	ml	-	-

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	29.10.21
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	n.d.
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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