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JCM 866 . DESULFOTOMACULUM BS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J866 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.30 g 0.318 2.34
K2HPO4 0.30 g 0.318 1.828
NH4Cl 1.00 g 1.062 19.846
NaCl 1.00 g 1.062 18.165
Na2SO4 3.00 g 3.185 22.421
MgCl2 x 6 H2O 0.50 g 0.531 2.611
CaCl2 x 2 H2O 0.10 g 0.106 0.722
KCl 0.10 g 0.106 1.424
Trace metal solution 1.00 ml - -
Resazurin 1.00 mg 0.001 0.005
Distilled water 940.00 ml - -
1 Mix components thoroughly, adjust pH to 7.2 with KOH, distribute the medium into culture vessels (e.g., 9.4 ml in Balch tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add the following solutions (per 9.4 ml medium, autoclaved or *filter-sterilized):
NaHCO3
(*, 8%) 		0.25 ml - -
Sodium pyruvate
(1 M) 		0.20 ml - -
Trace vitamins
(*) 		0.05 ml - -
Sodium dithionite
(freshly prepared, *, 1%) 		0.10 ml - -
Na2S x 9 H2O
(5%) 		0.04 ml - -

Trace metal solution #

1010
Solution occurs in 3 other media (first defined in Medium J294).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(32%) 		10.000 ml 3.168 86.898
FeCl2 x 4 H2O 2.000 g 1.98 9.96
CoCl2 x 6 H2O 0.250 g 0.248 1.906
MnCl2 x 4 H2O 0.100 g 0.099 0.5
ZnCl2 0.070 g 0.069 0.508
H3BO3 6.000 mg 0.006 0.096
Na2MoO4 x 2 H2O 0.040 g 0.04 0.164
NiCl2 x 6 H2O 0.070 g 0.069 0.535
CuCl2 x 2 H2O 2.000 mg 0.002 0.012
AlCl3 0.025 g 0.025 0.186
Na2WO4 x 2 H2O 6.000 mg 0.006 0.018
Distilled water 1000.000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 3.185 22.421
NH4Cl 1.062 19.846
NaCl 1.062 18.165
MgCl2 x 6 H2O 0.531 2.611
KH2PO4 0.318 2.34
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 2 strains
phyla 1 2 strains
nutrition chemolithoautotroph 2 strains
oxygen anaerobe 2 strains
temperature thermophilic 2 strains
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