J875
875 .
PHOSPHOAC3 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J875 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.282 | 2.074 | |
| K2HPO4 | 0.3 | g | 0.282 | 1.62 | |
| NH4Cl | 1.0 | g | 0.941 | 17.587 | |
| NaCl | 2.0 | g | 1.881 | 32.195 | |
| KCl | 0.1 | g | 0.094 | 1.262 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.094 | 0.64 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.47 | 2.314 | |
| Sodium acetate | 0.2 | g | 0.188 | 2.294 | |
| Trace minerals | 10.0 | ml | - | - | |
| Yeast extract | 2.0 | g | 1.881 | - | |
|
Sulfur
(powder) |
10.0 | g | 9.407 | 293.338 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.47 | - | |
| Resazurin | 1.0 | mg | 9.4e-4 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components except sulfur, and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium into culture vessels containing appropriate amount of sulfur while gassing with a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions (autoclaved and stored under N2, or *filter-sterilized and stored under N2-CO2, 4:1, v/v): | |||||
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
L-Sodium lactate
(1 M) |
20.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |