J876
876 .
DESULFONEMA MAGNUM MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J876 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 25.00 | g | 23.518 | 402.436 | |
| MgCl2 x 6 H2O | 5.60 | g | 5.268 | 25.913 | |
| MgSO4 x 7 H2O | 6.80 | g | 6.397 | 25.954 | |
| CaCl2 x 2 H2O | 1.40 | g | 1.317 | 8.958 | |
| KCl | 0.72 | g | 0.677 | 9.086 | |
| KH2PO4 | 0.14 | g | 0.132 | 0.968 | |
| NH4Cl | 0.25 | g | 0.235 | 4.397 | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Resazurin | 0.50 | mg | 4.7e-4 | 0.002 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly, bring to the boil and cool down under a N2-CO2 (4:1, v/v) gas mixture. After cooling, check pH to be around 6.0. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized and stored under N2) in the order listed: | |||||
|
Sodium benzoate
(6%) |
10.00 | ml | - | - | |
|
Trace vitamins (see Medium No. 197)
(*) |
10.00 | ml | - | - | |
|
AlK(SO4)2 x 12 H2O
(4.8%) |
10.00 | ml | - | - | |
|
Na2CO3
(0.5%) |
20.00 | ml | - | - | |
|
Na2S x 9 H2O
(3%) |
10.00 | ml | - | - | |
| 2 By the completion of the medium, white precipitate should be formed. Equilibrate the medium by standing the culture vessels overnight. | |||||
| 3 Comment: Addition of 10-20 mg/L sodium dithionate (filter-sterilized under N2) just before inoculation may stimulate growth at the beginning. Incubate with a 5-10% inoculum. | |||||
Solution occurs in 87
other media (first defined in Medium J187).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
| Distilled water | 990.0 | ml | - | - |
Solution occurs in 89
other media (first defined in Medium J187).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnCl2 | 70 | mg | 0.07 | 0.514 |
| MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
| H3BO3 | 6 | mg | 0.006 | 0.097 |
| CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
| CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
| NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
| Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 71
other media (first defined in Medium J431).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.4 | g | 0.4 | 10.001 |
| Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
| Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |