Loading ...

J881. KNH MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.136 g 0.136 0.996
2 0.535 g 0.533 9.972
3 3.050 g 3.041 14.958
4 0.147 g 0.147 0.997
5 2.520 g 2.512 29.908
6 20.450 g 20.389 348.87
7 2.840 g 2.832 19.934
8 0.656 g 0.654 7.973
9 Trace element solution 1.000 ml - -
10 Se/W solution 1.000 ml - -
11 Trace vitamins 1.000 ml - -
12 1.000 mg 1.0e-3 0.004
13 0.100 g 0.1 -
14 0.100 g 0.1 -
15 1000.000 ml 995.221 55243.1
Instructions:
  1. Mix all ingredients except sodium thioglycolate and ascorbic acid, and expel dissolving O2 from the solution with deoxygenated N2-CO2 (80:20, v/v). Dispense the solution into vials where deoxygenated H2-CO2 (80:20, v/v) is sparged. Cap with butyl rubber stoppers. Autoclave, then add reducing agent solution ( sodium thioglycolate and ascorbic acid, 1% solution each) sterilized with 0.2 �m-filter (0.1 ml in 10 ml medium) and inoculate fully grown culture at 5-10% of final volume. Fill with H2-CO2 (80:20, v/v) gas at 1.5 atm.
Solution occurs in 5 other media (first defined in medium J243 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 12.800 g 12.8 66.967
2 1.350 g 1.35 -
3 0.100 g 0.1 -
4 0.024 g 0.024 -
5 0.100 g 0.1 0.68
6 0.100 g 0.1 0.734
7 0.025 g 0.025 -
8 0.010 g 0.01 0.162
9 1.000 g 1 17.111
10 0.120 g 0.12 -
11 0.026 g 0.026 -
12 1000.000 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
  2. Comment: For strain JCM 10134, incubate with a 10-20% inoculum.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 8 other media (first defined in medium J284 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.004 -
2 4 mg 0.004 -
3 1000 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 20.39 348.887
MgCl2 x 6H2O 3.041 14.958
Na2SO4 2.832 19.934
NaHCO3 2.512 29.908
Show more

Similar media

J907. GEOBACTER MEDIUM 95 %
J807. WR MEDIUM FOR METHANOBACTERIACEAE 89 %
J1020. DESULFOCARBO INDIANENSIS MEDIUM 84 %
J243. METHANOTHRIX MEDIUM 83 %
J1258. DESULFOBACTERIUM OLEOVORANS MEDIUM 81 %

Latest comments

No comments for this medium.