J882
882 .
DETHIOSULFOVIBRIO BELB1 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J882 #
ml
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.298 | 2.187 | |
| K2HPO4 | 0.30 | g | 0.298 | 1.709 | |
| NH4Cl | 1.00 | g | 0.992 | 18.547 | |
| NaCl | 30.00 | g | 29.762 | 509.273 | |
| KCl | 0.10 | g | 0.099 | 1.331 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.099 | 0.675 | |
| Trace minerals | 10.00 | ml | - | - | |
| Sodium acetate | 0.05 | g | 0.05 | 0.605 | |
| Yeast extract | 0.10 | g | 0.099 | - | |
| Na2S2O3 x 5 H2O | 4.96 | g | 4.921 | 19.826 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.496 | - | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 915.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
25.00 | ml | - | - | |
|
MgCl2 x 6 H2O
(10%) |
30.00 | ml | - | - | |
|
Sodium acetate
(1.0 M) |
20.00 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere): | |||||
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |