Name: NATRONOLIMNOBIUS AHT32 MEDIUM (A)
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J887 #

Basal minerals #

54

Equipment needed: Autoclave Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂CO₃	190.0	g	3518.52	33197.3
NaHCO₃	30.0	g	555.556	6613.21
NaCl	16.0	g	296.296	5070.09
K₂HPO₄	2.0	g	37.037	212.641
1 Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Then, aseptically add per liter the following solutions (autoclaved or *filter-sterilized):
Trace vitamins (see Medium No. 197) (*)	10.0	ml	-	-
NH₄Cl (4 M)	1.0	ml	-	-
Selenite-tungstate solution (see Medium No. 431) (*)	1.0	ml	-	-
Trace element solution SL-4	1.0	ml	-	-
MgSO₄ (1 M)	1.0	ml	-	-
Yeast extract (10%)	0.2	ml	-	-
Sodium butyrate (1 M)	20.0	ml	-	-
Sodium acetate (1 M)	20.0	ml	-	-
2 Distribute the medium into culture vessels, replace the gas phase with N₂ containing 5% O₂ (by vol.) and close with butyl rubber stoppers.

1000

Solution occurs in 21 other media (first defined in Medium J340).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA	0.5	g	0.5	1.711
FeSO₄ x 7 H₂O	0.2	g	0.2	0.719
Trace element solution SL-6	100.0	ml	-	-
Distilled water	900.0	ml	-	-

1000

Solution occurs in 55 other media (first defined in Medium 27).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
MnCl₂ x 4 H₂O	0.03	g	0.03	0.152
H₃BO₃	0.30	g	0.3	4.851
CoCl₂ x 6 H₂O	0.20	g	0.2	1.54
CuCl₂ x 2 H₂O	0.01	g	0.01	0.059
NiCl₂ x 6 H₂O	0.02	g	0.02	0.154
Na₂MoO₄ x 2 H₂O	0.03	g	0.03	0.124
Distilled water	1000.00	ml	-	-