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JCM 889 . METALLIBACTERIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J889 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 132.0 mg 0.131 0.988
MgCl2 x 6 H2O 53.0 mg 0.052 0.258
KH2PO4 27.0 mg 0.027 0.196
CaCl2 x 2 H2O 147.0 mg 0.145 0.989
VOSO4 x n H2O 0.5 mg 4.9e-4 -
Wolfe's mineral elixir 1.0 ml - -
FeSO4 x 7 H2O 10.0 mg 0.01 0.036
Trypticase peptone
(BD-BBL) 		1.4 g 1.385 -
Trace vitamins 10.0 ml - -
Distilled water 1000.0 ml - -
1 Mix components, except Trace vitamins, adjust pH to 5.5 and autoclave. After cooling, aseptically add filter-sterilized Trace vitamins solution.

Wolfe's mineral elixir #

1000
Solution occurs in 8 other media (first defined in Medium J470).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x n H2O 5.00 g 5 -
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 -
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 24.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 1.385 -
CaCl2 x 2 H2O 0.146 0.996
(NH4)2SO4 0.131 0.988
MgCl2 x 6 H2O 0.052 0.258
MgSO4 x 7 H2O 0.03 0.12
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain