J892
892 .
MMJYP2 FOR AC55
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J892 #
ml
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 4.0 | g | 3.914 | - | |
|
Tryptone
(BD-Difco) |
1.0 | g | 0.978 | 13.766 | |
| Modified MJ synthetic seawater B | 1000.0 | ml | - | - | |
| 1 Mix components and adjust pH to 7.0. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions from anaerobic stocks (*filter-sterilized or autoclaved): | |||||
|
NaHCO3
(*, 8%) |
12.5 | ml | - | - | |
|
Na2S x 9 H2O
(pH 7.5, 5%) |
10.0 | ml | - | - | |
| 2 Pressurize the inoculated vessels to 200 kPa with the same gas mixture. | |||||
Solution occurs in 1
other media (first defined in Medium J892).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 25.00 | g | 24.753 | 423.554 |
| MgCl2 x 6 H2O | 4.20 | g | 4.158 | 20.454 |
| MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 |
| KCl | 0.50 | g | 0.495 | 6.641 |
| NH4Cl | 0.25 | g | 0.248 | 4.628 |
| K2HPO4 | 0.14 | g | 0.139 | 0.796 |
| CaCl2 x 2 H2O | 0.70 | g | 0.693 | 4.714 |
| NiCl2 x 6 H2O | 1.00 | mg | 9.9e-4 | 0.008 |
| Na2SeO3 x 5 H2O | 1.00 | mg | 9.9e-4 | 0.004 |
| Trace mineral solution | 10.00 | ml | - | - |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 1
other media (first defined in Medium J892).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Trace minerals | 1000.000 | ml | - | - |
| NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 |
| Na2SeO3 x 5 H2O | 0.300 | mg | 3.0e-4 | 0.001 |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 24.229 | 414.602 |
| MgCl2 x 6 H2O | 4.069 | 20.014 |
| Yeast extract | 3.914 | - |
| MgSO4 x 7 H2O | 3.323 | 13.482 |
| Tryptone | 0.978 | 13.766 |
| Show more | ||
