Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.20 | g | 0.198 | 1.458 | |
NH4Cl | 0.25 | g | 0.248 | 4.637 | |
NaCl | 1.00 | g | 0.992 | 16.976 | |
MgCl2 x 6 H2O | 0.40 | g | 0.397 | 1.952 | |
KCl | 0.50 | g | 0.496 | 6.654 | |
CaCl2 x 2 H2O | 0.10 | g | 0.099 | 0.675 | |
NaHCO3 | 0.10 | g | 0.099 | 1.181 | |
Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
Yeast extract
(BD-Difco) |
2.00 | g | 1.984 | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components thoroughly and autoclave under a N2 gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
Vitamin solution
(*) |
1.00 | ml | - | - | |
Trace element solution
(*) |
1.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
6.00 | ml | - | - | |
2 Check pH to be 7.5 - 7.6. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
p-Aminobenzoic acid | 25.0 | mg | 0.025 | 0.182 |
Biotin | 100.0 | mg | 0.1 | 0.409 |
Nicotinic acid | 25.0 | mg | 0.025 | 0.203 |
DL-Calcium pantothenate | 25.0 | mg | 0.025 | 0.052 |
Pyridoxine hydrochloride | 25.0 | mg | 0.025 | - |
Folic acid | 10.0 | mg | 0.01 | 0.023 |
Riboflavin | 25.0 | mg | 0.025 | 0.066 |
Vitamin B12 | 0.5 | mg | 5.0e-4 | 3.7e-4 |
Lipoic acid | 25.0 | mg | 0.025 | 0.121 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Fe(NH4)2(SO4)2 x 6 H2O | 784 | mg | 0.784 | - |
CoCl2 x 6 H2O | 238 | mg | 0.238 | 1.833 |
(NH4)2Ni(SO4)2 x 6 H2O | 395 | mg | 0.395 | 1 |
Na2MoO4 x 2 H2O | 24 | mg | 0.024 | 0.099 |
Na2WO4 x 2 H2O | 33 | mg | 0.033 | 0.1 |
ZnSO4 x 7 H2O | 144 | mg | 0.144 | 0.501 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
Na2SeO4 | 94 | mg | 0.094 | 0.497 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
MnCl2 x 4 H2O | 99 | mg | 0.099 | - |
Distilled water | 1000 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Yeast extract | 1.984 | - |
NaCl | 0.992 | 16.976 |
KCl | 0.496 | 6.654 |
MgCl2 x 6 H2O | 0.397 | 1.952 |
NH4Cl | 0.248 | 4.637 |
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