Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trisodium citrate x 2 H2O | 1.00 | g | 9.901 | - | |
Tryptone
(BD-Difco) |
2.00 | g | 19.802 | 278.587 | |
Peptone
(Oxoid) |
1.00 | g | 9.901 | - | |
KH2PO4 | 0.60 | g | 5.941 | 43.653 | |
NH4Cl | 0.60 | g | 5.941 | 111.06 | |
NaCl | 200.00 | g | 1980.2 | 33884.3 | |
MgCl2 x 6 H2O | 0.20 | g | 1.98 | 9.74 | |
K2SO4 | 20.00 | g | 198.02 | 1136.35 | |
FeCl2 x 4 H2O | 36.00 | mg | 0.356 | 1.793 | |
MnCl2 x 4 H2O | 0.36 | mg | 0.004 | - | |
SL-6 trace element solution | 1.00 | ml | - | - | |
1 Add components to distilled water and bring volume to 900 ml. Adjust pH to 7.0 and autoclave. For preparation of solid medium, add 20.0 g/L Bacto agar (BD-Difco). Separately autoclave the following solution: | |||||
Glycine-NaOH buffer
(pH 10.0, 0.5 M) |
100.00 | ml | - | - | |
2 After autoclaving, add the solution to the medium. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
H3BO3 | 0.30 | g | 0.3 | 4.851 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 3.6. |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 1980.2 | 33884.3 |
K2SO4 | 198.02 | 1136.35 |
Tryptone | 19.802 | 278.587 |
Trisodium citrate x 2 H2O | 9.901 | - |
Peptone | 9.901 | - |
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