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J907. GEOBACTER MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.136 g 0.133 0.979
2 0.535 g 0.524 9.796
3 0.204 g 0.2 0.983
4 0.147 g 0.144 0.979
5 0.820 g 0.803 9.79
6 0.142 g 0.139 0.979
7 Trace element solution 10.000 ml - -
8 Trace vitamins 10.000 ml - -
9 Se/W solution 1.000 ml - -
10 920.000 ml 899.462 49927.7
11
(filter-sterilized, 8%)
30.000 ml 5.075 60.412
12 100 mM Fe NTA solution
(100 mM)
50.000 ml - -
Instructions:
  1. Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. After cooling, add aseptically and anaerobically the following solutions to the medium: #11-12
  2. Adjust pH to 7.0 - 7.2, if necessary.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.64 g - -
2 2.75 g - -
3 2.70 g - -
Instructions:
  1. Firstly, dissolve NaHCO3 in 80 ml distilled water. Then, add NTA and FeCl3 x 6H2O to the solution and bring volume to 100 ml with distilled water. Adjust pH to 7.0 with NaOH. Filter-sterilize and store under a N2 atmosphere.
  2. Comment:
  3. 100 mM Fe NTA solution can be replaced with Iron(III)citrate solution (see Medium No. 1014, autoclaved and stored under N2).
Solution occurs in 5 other media (first defined in medium J243 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 12.800 g 12.8 66.967
2 1.350 g 1.35 -
3 0.100 g 0.1 -
4 0.024 g 0.024 -
5 0.100 g 0.1 0.68
6 0.100 g 0.1 0.734
7 0.025 g 0.025 -
8 0.010 g 0.01 0.162
9 1.000 g 1 17.111
10 0.120 g 0.12 -
11 0.026 g 0.026 -
12 1000.000 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
  2. Comment: For strain JCM 10134, incubate with a 10-20% inoculum.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 8 other media (first defined in medium J284 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.004 -
2 4 mg 0.004 -
3 1000 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 919.993 51067.4
NaHCO3 5.075 60.412
Sodium acetate 0.803 9.79
NH4Cl 0.524 9.796
MgCl2 x 6H2O 0.2 0.983
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