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JCM 907 . GEOBACTER MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J907 #

ml
Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.136 g 0.133 0.979
NH4Cl 0.535 g 0.524 9.796
MgCl2 x 6 H2O 0.204 g 0.2 0.983
CaCl2 x 2 H2O 0.147 g 0.144 0.979
Sodium acetate 0.820 g 0.803 9.791
Na2SO4 0.142 g 0.139 0.979
Trace element solution 10.000 ml - -
Trace vitamins 10.000 ml - -
Se/W solution 1.000 ml - -
Distilled water 920.000 ml - -
1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. After cooling, add aseptically and anaerobically the following solutions to the medium:
NaHCO3
(filter-sterilized, 8%) 		30.000 ml - -
100 mM Fe NTA solution
(100 mM) 		50.000 ml - -
2 Adjust pH to 7.0 - 7.2, if necessary.

100 mM Fe NTA solution #

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1.64 g - -
Nitrilotriacetic acid trisodium salt monohydrate 2.75 g - -
FeCl3 x 6 H2O 2.70 g - -
1 Firstly, dissolve NaHCO3 in 80 ml distilled water. Then, add NTA and FeCl3 x 6H2O to the solution and bring volume to 100 ml with distilled water. Adjust pH to 7.0 with NaOH. Filter-sterilize and store under a N2 atmosphere.
2 Comment:
3 100 mM Fe NTA solution can be replaced with Iron(III)citrate solution (see Medium No. 1014, autoclaved and stored under N2).

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium J243).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
NaCl 1.000 g 1 17.112
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Na2SeO3 x 5 H2O 0.026 g 0.026 0.099
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
2 Comment: For strain JCM 10134, incubate with a 10-20% inoculum.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Se/W solution #

1000
Solution occurs in 8 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 4 mg 0.004 0.015
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium acetate 0.803 9.791
NH4Cl 0.524 9.796
MgCl2 x 6 H2O 0.2 0.983
CaCl2 x 2 H2O 0.145 0.986
Na2SO4 0.139 0.979
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
phyla 2 3 strains
oxygen anaerobe 2 strains
temperature mesophilic 3 strains