Name: IRD MARINE CLOSTRIDA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J909 #

Equipment needed: Filter Autoclave Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.3	g	0.295	2.166
K₂HPO₄	0.3	g	0.295	1.692
NH₄Cl	1.0	g	0.982	18.365
NaCl	23.0	g	22.593	386.607
KCl	0.1	g	0.098	1.318
CaCl₂ x 2 H₂O	0.1	g	0.098	0.668
Trace minerals	10.0	ml	-	-
Yeast extract	0.5	g	0.491	-
L-Cysteine HCl x H₂O	0.5	g	0.491	-
Resazurin	1.0	mg	9.8e-4	0.004
Distilled water	940.0	ml	-	-
1 Mix components thoroughly and autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO₃ (*, 8%)	25.0	ml	-	-
MgCl₂ x 6 H₂O (10%)	30.0	ml	-	-
Glucose (1 M)	5.0	ml	-	-
2 Aseptically distribute the medium into culture vessels under a stream of N₂-CO₂ (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N₂ atmosphere):
Na₂S x 9 H₂O (5%)	8.0	ml	-	-

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	29.10.21
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	n.d.
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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