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JCM 911 . METHANOCELLA CONRADII MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J911 #

ml
Equipment needed: Autoclave Hungate tubes Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.500 g 0.48 6.443
MgCl2 x 6 H2O 0.400 g 0.384 1.89
KH2PO4 0.200 g 0.192 1.412
NH4Cl 0.100 g 0.096 1.796
CaCl2 x 2 H2O 0.100 g 0.096 0.653
Sodium acetate x 3 H2O 0.082 g 0.079 -
L-Cysteine HCl x H2O 0.300 g 0.288 -
Yeast extract 0.200 g 0.192 -
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Tungstate solution 1.000 ml - -
Resazurin 0.500 mg 4.8e-4 0.002
Distilled water 1000.000 ml - -
1 Mix components thoroughly, bring to a boil and cool down under a stream of N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically, add per liter the following solutions (filter-sterilized):
NaHCO3
(8%) 		30.000 ml - -
Vitamin solution 1.000 ml - -
Thiamine solution 1.000 ml - -
Vitamin B12 solution 0.05 g/L 1.000 ml - -
2 Prior to inoculation, replace the gas phase with a H2-CO2 (4:1, v/v) gas mixture and add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 gas atmosphere):
Na2S x 9 H2O
(5%) 		5.000 ml - -
3 Pressurize the inoculate culture vessels to 150 kPa H2-CO2 (4:1, v/v).

Tungstate solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2WO4 x 2 H2O 7.0 mg 0.007 0.021
Distilled water 1000.0 ml - -
1 Comment: For strain JCM 17849, inoculate with a 10% inoculum. When the cellular density increases, re-pressure the culture vessels to 150 kPa with the same gas mixture.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Vitamin solution #

100
Solution occurs in 14 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 4 mg 0.04 0.292
Biotin 1 mg 0.01 0.041
Nicotinic acid 10 mg 0.1 0.812
DL-Calcium pantothenate 5 mg 0.05 0.105
Pyridoxine hydrochloride 10 mg 0.1 -
Sodium phosphate buffer
(10 mM, pH 7.1) 		100 ml - -

Thiamine solution #

100
Solution occurs in 10 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine HCl 10 mg 0.1 -
Sodium phosphate buffer
(25 mM, pH 3.4) 		100 ml - -

Vitamin B12 solution 0.05 g/L #

100
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KCl 0.48 6.443
MgCl2 x 6 H2O 0.384 1.89
L-Cysteine HCl x H2O 0.288 -
KH2PO4 0.192 1.412
Yeast extract 0.192 -
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