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J911. METHANOCELLA CONRADII MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.500 g 0.48 6.443
2 0.400 g 0.384 1.89
3 0.200 g 0.192 1.412
4 0.100 g 0.096 1.796
5 0.100 g 0.096 0.653
6 0.082 g 0.079 -
7 0.300 g 0.288 1.641
8 0.200 g 0.192 -
9 FeCl2 solution 1.000 ml - -
10 Trace element solution 1.000 ml - -
11 Tungstate solution 1.000 ml - -
12 0.500 mg 4.8e-4 0.002
13 1000.000 ml 958.892 53226.6
14
(8%)
30.000 ml 4.978 59.252
15 Vitamin solution 1.000 ml - -
16 Thiamine solution 1.000 ml - -
17 Vitamin B12 solution 1.000 ml - -
18
(5%)
5.000 ml - -
Instructions:
  1. Mix components thoroughly, bring to a boil and cool down under a stream of N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically, add per liter the following solutions (filter-sterilized): #14-17
  2. Prior to inoculation, replace the gas phase with a H2-CO2 (4:1, v/v) gas mixture and add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 gas atmosphere): #18
  3. Pressurize the inoculate culture vessels to 150 kPa H2-CO2 (4:1, v/v).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 7.0 mg 0.007 -
3 1000.0 ml 998.207 55408.8
Instructions:
  1. Comment: For strain JCM 17849, inoculate with a 10% inoculum. When the cellular density increases, re-pressure the culture vessels to 150 kPa with the same gas mixture.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 14 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.04 0.292
2 1 mg 0.01 0.041
3 10 mg 0.1 0.812
4 5 mg 0.05 -
5 10 mg 0.1 -
6
(10 mM, pH 7.1)
100 ml - -
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 10 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 mg 0.1 -
2
(25 mM, pH 3.4)
100 ml - -
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 962.718 53439
NaHCO3 4.978 59.252
KCl 0.48 6.443
MgCl2 x 6H2O 0.384 1.89
L-Cysteine x HCl x H2O 0.288 1.641
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