Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract
(BD-Difco) |
0.20 | g | 0.195 | - | |
KH2PO4 | 0.14 | g | 0.137 | 1.004 | |
MgCl2 x 6 H2O | 0.20 | g | 0.195 | 0.96 | |
CaCl2 x 2 H2O | 0.15 | g | 0.146 | 0.995 | |
NH4Cl | 0.54 | g | 0.527 | 9.849 | |
Trace vitamins solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Se/W solution | 1.00 | ml | - | - | |
NaHCO3 | 2.50 | g | 2.439 | 29.034 | |
Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components, except NaHCO3, and adjust pH to 6.5. Bring to a boil for 5-10 sec, cool down under a stream of a N2-CO2 (80:20 v/v) gas mixture and add NaHCO3 to the solution. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottle) under the same gas mixture and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Then, add per liter the following solutions: | |||||
Glucose
(1.0 M) |
10.00 | ml | - | - | |
L-Cysteine HCl x H2O
(5%) |
6.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
6.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 4.9 | mg | 0.005 | 0.02 |
Folic acid | 8.8 | mg | 0.009 | 0.02 |
Pyridoxine hydrochloride | 4.1 | mg | 0.004 | - |
Thiamine HCl | 6.7 | mg | 0.007 | - |
Riboflavin | 7.5 | mg | 0.008 | 0.02 |
Nicotinic acid | 2.5 | mg | 0.003 | 0.02 |
DL-Calcium pantothenate | 9.5 | mg | 0.01 | 0.02 |
Vitamin B12 | 27.1 | mg | 0.027 | 0.02 |
p-Aminobenzoic acid | 2.7 | mg | 0.003 | 0.02 |
Lipoic acid | 4.1 | mg | 0.004 | 0.02 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 12.800 | g | 12.8 | 66.967 | |
FeCl3 x 6 H2O | 1.350 | g | 1.35 | 4.995 | |
MnCl2 x 4 H2O | 0.100 | g | 0.1 | - | |
CoCl2 x 6 H2O | 0.024 | g | 0.024 | 0.185 | |
CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
ZnCl2 | 0.100 | g | 0.1 | 0.734 | |
CuCl2 x 2 H2O | 0.025 | g | 0.025 | 0.147 | |
H3BO3 | 0.010 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.024 | g | 0.024 | 0.099 | |
NiCl2 x 6 H2O | 0.120 | g | 0.12 | 0.926 | |
Distilled water | 1000.000 | ml | - | - | |
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2SeO3 x 5 H2O | 4 | mg | 0.004 | 0.015 |
Na2WO4 x 2 H2O | 4 | mg | 0.004 | 0.012 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.5 |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 2.439 | 29.034 |
NH4Cl | 0.527 | 9.849 |
MgCl2 x 6 H2O | 0.195 | 0.96 |
Yeast extract | 0.195 | - |
CaCl2 x 2 H2O | 0.146 | 0.996 |
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