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JCM 924 . PEAT MEDIUM 2 FOR METHANOBACTERIA

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J924 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Major metals 10.000 ml - -
Trace metal 2 solution 1.000 ml - -
Distilled water 1000.000 ml - -
1 To complete the medium, add the following solutions to each of culture tubes (containing 5 ml medium) from sterile anaerobic stocks (autoclaved or *filter-sterilized):
83 mM TiNTA solution
(*, 83 mM) 		0.050 ml - -
MES
(pH 7.45, *, 1.0 M) 		0.100 ml - -
Vitamin solution
(*) 		0.050 ml - -
Yeast extract
(1%) 		0.010 ml - -
Coenzyme M
(50 mM) 		0.025 ml - -
Sodium acetate
(10 mM) 		0.015 ml - -
Na2S x 9 H2O
(*, 4 mM) 		0.050 ml - -
2 Mix components thoroughly, bring to a boil for 5 min, then, cool down under a stream of a N2-CO2 (80:20, v/v) gas mixture. Distribute the medium into Balch-type culture tubes (5 ml each) under the same gas mixture, seal with thick blue butyl rubber stoppers and autoclave.
3 Pressurize the tubes to 70 kPa H2-CO2 (80:20, v/v) and allow to stand for at least 7 hr. Check the final pH of the medium to be about 5.7.

Trace metal 2 solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace metal 1 solution 1000.00 ml - -
Na2-EDTA 37.23 g 37.23 -
1 Adjust pH to 7.0 with NaOH.

Major metals #

1000
Solution occurs in 2 other media (first defined in Medium J923).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.15 g 0.15 2.012
KH2PO4 1.36 g 1.36 9.994
NH4Cl 2.68 g 2.68 50.103
Distilled water 1000.00 ml - -

83 mM TiNTA solution #

13
Solution occurs in 1 other media (first defined in Medium J923).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tris base
(pH 8, 1 M) 		7.20 ml - -
Nitrilotriacetic acid , disodium salt
(0.5 M) 		4.80 ml - -
TiCl3
(15%) 		0.55 ml - -

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium J923).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 -
Thiamine HCl 50 mg 0.05 -
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
DL-Calcium pantothenate 50 mg 0.05 0.105
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -
1 Filter-sterilize and store under N2 at 4C in the dark.

Trace metal 1 solution #

1000
Solution occurs in 2 other media (first defined in Medium J923).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CoCl2 x 6 H2O 24.000 mg 0.024 0.185
ZnCl2 75.000 mg 0.075 0.55
H3BO3 19.000 mg 0.019 0.307
NiCl2 x 6 H2O 24.000 mg 0.024 0.185
Na2MoO4 x 2 H2O 24.000 mg 0.024 0.099
FeCl2 x 4 H2O 1.344 g 1.344 6.76
MnSO4 x n H2O 26.000 mg 0.026 -
MgSO4 x 7 H2O 1.556 g 1.556 6.313
CaCl2 x 2 H2O 2.336 g 2.336 15.89
CuSO4 x 5 H2O 9.000 mg 0.009 0.036
AlK(SO4)2 x 12 H2O 3.446 g 3.446 7.264
Distilled water 1000.000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2-EDTA 0.037 -
NH4Cl 0.027 0.496
KH2PO4 0.013 0.099
AlK(SO4)2 x 12 H2O 0.003 0.007
CaCl2 x 2 H2O 0.002 0.016
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature psychrophilic 1 strain