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J928. METHYLOBACTER MR1 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 19.901 340.514
2 0.09 g 0.09 0.658
3 0.26 g 0.259 1.822
4 0.50 g 0.498 3.384
5 3.40 g 3.383 13.726
6 4.20 g 4.179 20.556
7 0.33 g 0.328 4.404
8 0.25 g 0.249 2.927
9 0.25 g 0.249 4.65
10 0.02 g 0.02 0.072
11 Trace mineral solution 3.00 ml - -
12
(1 mM)
0.10 ml - -
13 1000.00 ml 993.241 55133.2
14 Trace vitamins 1.00 ml - -
15
(8%)
1.25 ml 0.215 2.557
Instructions:
  1. Mix components, adjust pH to 6.6 - 6.8 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium: #14-15
  2. Distribute the medium into culture vessels (leave >80% volume gas phase), purge air in the gas phase with N2 for 1 min and seal with butyl rubber stoppers. Inject O2 gas into the gas phase (1.5% volume of the gas phase). Then, overpressure the culture vessels with methane gas (50% volume of the gas phase).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 0.50 g 0.5 -
3 0.10 g 0.1 0.36
4 0.18 g 0.18 -
5 0.18 g 0.18 -
6 0.03 g 0.03 0.124
7 0.02 g 0.02 -
8 0.01 g 0.01 0.162
9 0.02 g 0.02 -
10 0.01 g 0.01 -
11 5.00 mg 0.005 -
12 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then, proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.214 55353.7
NaCl 19.901 340.514
MgCl2 x 6H2O 4.179 20.556
MgSO4 x 7H2O 3.383 13.726
CaCl2 x 2H2O 0.498 3.384
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