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JCM 928 . METHYLOBACTER MR1 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J928 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.901 340.529
KH2PO4 0.09 g 0.09 0.658
Na2HPO4 0.26 g 0.259 1.822
CaCl2 x 2 H2O 0.50 g 0.498 3.384
MgSO4 x 7 H2O 3.40 g 3.383 13.726
MgCl2 x 6 H2O 4.20 g 4.179 20.556
KCl 0.33 g 0.328 4.405
NaNO3 0.25 g 0.249 2.927
NH4Cl 0.25 g 0.249 4.651
FeSO4 x 7 H2O 0.02 g 0.02 0.072
Trace mineral solution 3.00 ml - -
CuSO4 x 5 H2O
(1 mM) 		0.10 ml - -
Distilled water 1000.00 ml - -
1 Mix components, adjust pH to 6.6 - 6.8 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium:
Trace vitamins 1.00 ml - -
NaHCO3
(8%) 		1.25 ml - -
2 Distribute the medium into culture vessels (leave >80% volume gas phase), purge air in the gas phase with N2 for 1 min and seal with butyl rubber stoppers. Inject O2 gas into the gas phase (1.5% volume of the gas phase). Then, overpressure the culture vessels with methane gas (50% volume of the gas phase).

Trace mineral solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x n H2O 0.50 g 0.5 -
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2WO4 x 2 H2O 0.02 g 0.02 0.061
NiCl2 x 6 H2O 0.01 g 0.01 0.077
Na2SeO3 x 5 H2O 5.00 mg 0.005 0.019
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then, proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.901 340.529
MgCl2 x 6 H2O 4.179 20.556
MgSO4 x 7 H2O 3.383 13.726
CaCl2 x 2 H2O 0.498 3.384
KCl 0.328 4.405
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