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JCM 933 . THERMOANAEROBACULUM AQUATICUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J933 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.25 g 0.246 1.67
KH2PO4 0.34 g 0.334 2.454
NH4Cl 0.34 g 0.334 6.244
KCl 0.34 g 0.334 4.48
MgSO4 x 7 H2O 0.50 g 0.491 1.993
NaCl 0.25 g 0.246 4.202
Yeast extract 0.10 g 0.098 -
RST trace elements 1.00 ml - -
Coenzyme M
(0.001%) 		0.50 ml - -
Trace vitamins 1.00 ml - -
Sodium nitrate 0.85 g 0.835 9.824
Sodium pyruvate 1.10 g 1.081 9.82
TES 1.00 g 0.982 4.285
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components thoroughly and adjust pH to 7.0 with NaOH. Distribute the medium into culture vessels under a N2 gas stream, seal with butyl rubber stoppers and autoclave. Reduce the medium by the addition of the following solutions (autoclaved under a N2 gas atmosphere) per liter:
Na2S x 9 H2O
(5%) 		8.00 ml - -
L-Cysteine HCl x H2O
(5%) 		8.00 ml - -

RST trace elements #

1000
Solution occurs in 1 other media (first defined in Medium J933).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 2.00 g 2 10.464
MnSO4 x n H2O 1.00 g 1 -
Fe(NH4)2(SO4)2 x 6 H2O 0.80 g 0.8 -
CoCl2 x 6 H2O 0.20 g 0.2 1.54
ZnSO4 x 7 H2O 0.20 g 0.2 0.696
CuCl2 x 2 H2O 0.02 g 0.02 0.117
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Na2SeO4 0.02 g 0.02 0.106
Na2WO4 x 2 H2O 0.02 g 0.02 0.061
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.0 with KOH. Then proceed to add minerals.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium pyruvate 1.081 9.82
TES 0.982 4.285
Sodium nitrate 0.835 9.824
MgSO4 x 7 H2O 0.491 1.993
NH4Cl 0.334 6.244
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain