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J935. FERVIDICOCCUS FONTIS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.315 5.892
2 0.33 g 0.315 2.316
3 0.33 g 0.315 4.228
4 0.44 g 0.42 2.859
5 0.70 g 0.669 3.289
6 0.50 g 0.478 8.171
7 0.80 g 0.764 9.096
8 FeCl2 solution 1.00 ml - -
9 Trace element solution 1.00 ml - -
10 1.00 mg 9.6e-4 0.004
11 1000.00 ml 953.397 52921.5
12 This solution has been removed!
(w/v) Yeast extract (BD-Difco, 10%)
5.00 ml - -
13 This solution has been removed!
(w/v) Trypticase peptone (BD-BBL, 10%)
20.00 ml - -
14 Trace vitamins
(*)
10.00 ml - -
15
(w/v, 5%)
10.00 ml - -
Instructions:
  1. Mix components thoroughly, adjust pH to 5.0 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): #12-15
  2. Readjust pH to 5.5 - 6.0 with sterile 1 N H2SO4, if necessary.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 964.828 53556
NaHCO3 0.764 9.096
MgCl2 x 6H2O 0.669 3.289
NaCl 0.478 8.171
CaCl2 x 2H2O 0.42 2.859
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