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JCM 935 . FERVIDICOCCUS FONTIS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J935 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.315 5.892
KH2PO4 0.33 g 0.315 2.316
KCl 0.33 g 0.315 4.228
CaCl2 x 2 H2O 0.44 g 0.42 2.859
MgCl2 x 6 H2O 0.70 g 0.669 3.289
NaCl 0.50 g 0.478 8.172
NaHCO3 0.80 g 0.764 9.096
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Resazurin 1.00 mg 9.6e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components thoroughly, adjust pH to 5.0 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):

(w/v) Yeast extract (BD-Difco, 10%) 		5.00 ml - -

(w/v) Trypticase peptone (BD-BBL, 10%) 		20.00 ml - -
Trace vitamins
(*) 		10.00 ml - -
Na2S x 9 H2O
(w/v, 5%) 		10.00 ml - -
2 Readjust pH to 5.5 - 6.0 with sterile 1 N H2SO4, if necessary.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 03.04.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.8
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 0.764 9.096
MgCl2 x 6 H2O 0.669 3.289
NaCl 0.478 8.172
CaCl2 x 2 H2O 0.42 2.859
NH4Cl 0.315 5.892
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
nutrition organotroph 1 strain
oxygen anaerobe 1 strain
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