Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.33 | g | 0.315 | 5.892 | |
KH2PO4 | 0.33 | g | 0.315 | 2.316 | |
KCl | 0.33 | g | 0.315 | 4.228 | |
CaCl2 x 2 H2O | 0.44 | g | 0.42 | 2.859 | |
MgCl2 x 6 H2O | 0.70 | g | 0.669 | 3.289 | |
NaCl | 0.50 | g | 0.478 | 8.172 | |
NaHCO3 | 0.80 | g | 0.764 | 9.096 | |
FeCl2 solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Resazurin | 1.00 | mg | 9.6e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components thoroughly, adjust pH to 5.0 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
(w/v) Yeast extract (BD-Difco, 10%) |
5.00 | ml | - | - | |
(w/v) Trypticase peptone (BD-BBL, 10%) |
20.00 | ml | - | - | |
Trace vitamins
(*) |
10.00 | ml | - | - | |
Na2S x 9 H2O
(w/v, 5%) |
10.00 | ml | - | - | |
2 Readjust pH to 5.5 - 6.0 with sterile 1 N H2SO4, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 03.04.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 5.8 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 0.764 | 9.096 |
MgCl2 x 6 H2O | 0.669 | 3.289 |
NaCl | 0.478 | 8.172 |
CaCl2 x 2 H2O | 0.42 | 2.859 |
NH4Cl | 0.315 | 5.892 |
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