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JCM 936 . LX-D MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J936 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD-Difco) 		0.20 g 0.195 -
KH2PO4 0.14 g 0.137 1.004
MgCl2 x 6 H2O 0.20 g 0.195 0.96
CaCl2 x 2 H2O 0.15 g 0.146 0.995
NH4Cl 0.54 g 0.527 9.849
Trace vitamins solution 1.00 ml - -
Trace element solution 1.00 ml - -
Se/W solution 1.00 ml - -
NaHCO3 2.50 g 2.439 29.034
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components, except NaHCO3, and adjust pH to 6.5. Bring to a boil for 5-10 sec, cool down under a stream of a N2-CO2 (80:20 v/v) gas mixture and add NaHCO3 to the solution. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottle) under the same gas mixture and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Then, add per liter the following solutions:
Methanol
(1.0 M) 		10.00 ml - -
L-Cysteine HCl x H2O
(5%) 		6.00 ml - -
Na2S x 9 H2O
(5%) 		6.00 ml - -

Trace vitamins solution #

1000
Solution occurs in 9 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.9 mg 0.005 0.02
Folic acid 8.8 mg 0.009 0.02
Pyridoxine hydrochloride 4.1 mg 0.004 -
Thiamine HCl 6.7 mg 0.007 -
Riboflavin 7.5 mg 0.008 0.02
Nicotinic acid 2.5 mg 0.003 0.02
DL-Calcium pantothenate 9.5 mg 0.01 0.02
Vitamin B12 27.1 mg 0.027 0.02
p-Aminobenzoic acid 2.7 mg 0.003 0.02
Lipoic acid 4.1 mg 0.004 0.02
Distilled water 1000.0 ml - -

Trace element solution #

1000
Solution occurs in 7 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH.

Se/W solution #

1000
Solution occurs in 8 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 4 mg 0.004 0.015
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 6.5
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.439 29.034
NH4Cl 0.527 9.849
MgCl2 x 6 H2O 0.195 0.96
Yeast extract 0.195 -
CaCl2 x 2 H2O 0.146 0.996
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