Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.300 | g | 0.295 | 2.166 | |
K2HPO4 | 0.300 | g | 0.295 | 1.692 | |
NH4Cl | 0.500 | g | 0.491 | 9.182 | |
NaCl | 5.000 | g | 4.912 | 84.045 | |
KCl | 0.100 | g | 0.098 | 1.318 | |
CaCl2 x 2 H2O | 0.100 | g | 0.098 | 0.668 | |
MgCl2 x 6 H2O | 0.500 | g | 0.491 | 2.416 | |
Sodium acetate | 0.164 | g | 0.161 | 1.964 | |
Trace minerals | 10.000 | ml | - | - | |
Yeast extract | 5.000 | g | 4.912 | - | |
Trypticase peptone
(BD-BBL) |
5.000 | g | 4.912 | - | |
L-Cysteine HCl x H2O | 0.500 | g | 0.491 | - | |
Resazurin | 1.000 | mg | 9.8e-4 | 0.004 | |
Distilled water | 885.000 | ml | - | - | |
1 Mix components thoroughly, adjust pH to 8.5 and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
NaHCO3
(*, 8%) |
75.000 | ml | - | - | |
Crotonic acid
(pH 7.0, 0.025 M) |
40.000 | ml | - | - | |
2 Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere): | |||||
Na2S x 9 H2O
(5%) |
8.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 8.5 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 4.921 | 84.213 |
Yeast extract | 4.912 | - |
Trypticase peptone | 4.912 | - |
NH4Cl | 0.491 | 9.182 |
MgCl2 x 6 H2O | 0.491 | 2.416 |
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