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JCM 940 . AMINIVIBRIO ASINOENSIS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J940 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.192 1.412
NH4Cl 0.25 g 0.24 4.49
NaCl 1.00 g 0.961 16.438
KCl 0.50 g 0.48 6.443
MgCl2 x 6 H2O 0.40 g 0.384 1.89
CaCl2 x 2 H2O 0.15 g 0.144 0.98
Sodium pyruvate 1.10 g 1.057 9.603
Yeast extract 2.00 g 1.921 -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Resazurin 0.40 mg 3.8e-4 0.002
Distilled water 1000.00 ml - -
1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (filter-sterilized):
NaHCO3
(8%) 		30.00 ml - -
Vitamin solution 1.00 ml - -
2 Distribute the medium into culture vessels under the same gas mixture. Finally, add per liter the following solution from an anaerobic stock:
Na2S x 9 H2O
(5%) 		7.00 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 80 mg 0.08 0.583
Biotin 20 mg 0.02 0.082
Thiamine HCl 200 mg 0.2 -
Vitamin B12 50 mg 0.05 0.037
Distilled water 1000 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.921 -
Sodium pyruvate 1.057 9.603
NaCl 0.961 16.438
KCl 0.48 6.443
MgCl2 x 6 H2O 0.384 1.89
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain