Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 1.00 | g | 0.988 | 16.909 | |
KCl | 0.50 | g | 0.494 | 6.627 | |
MgCl2 x 6 H2O | 0.40 | g | 0.395 | 1.944 | |
NH4Cl | 0.25 | g | 0.247 | 4.618 | |
CaCl2 x 2 H2O | 0.15 | g | 0.148 | 1.008 | |
Trace elements solution | 10.00 | ml | - | - | |
Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
Distilled water | 946.00 | ml | - | - | |
1 Mix components thoroughly, and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
KH2PO4
(10%) |
2.00 | ml | - | - | |
NaHCO3
(*, 8%) |
32.00 | ml | - | - | |
Vitamin solution
(*) |
10.00 | ml | - | - | |
2 Aseptically distribute the medium into culture vessels under a H2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers (leave >80% gas phase by volume). Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere): | |||||
L-Cysteine HCl x H2O
(5%) |
6.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
6.00 | ml | - | - | |
3 Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20, v/v). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
Fe(NH4)2(SO4)2 x 6 H2O | 0.20 | g | 0.2 | - | |
Na2SeO3 x 5 H2O | 0.20 | g | 0.2 | 0.76 | |
MnCl2 x 4 H2O | 0.11 | g | 0.11 | - | |
CoCl2 x 6 H2O | 0.10 | g | 0.1 | 0.77 | |
Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
Na2WO4 x 2 H2O | 0.10 | g | 0.1 | - | |
NiCl2 x 6 H2O | 0.25 | g | 0.25 | 1.929 | |
ZnSO4 x 7 H2O | 0.20 | g | 0.2 | 0.696 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
CuSO4 x 5 H2O | 2.00 | mg | 0.002 | 0.008 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
p-Aminobenzoic acid | 10 | mg | 0.01 | 0.073 |
Nicotinic acid | 10 | mg | 0.01 | 0.081 |
DL-Calcium pantothenate | 10 | mg | 0.01 | 0.021 |
Pyridoxine hydrochloride | 10 | mg | 0.01 | - |
Riboflavin | 10 | mg | 0.01 | 0.027 |
Thiamine HCl | 10 | mg | 0.01 | - |
Biotin | 5 | mg | 0.005 | 0.02 |
Folic acid | 5 | mg | 0.005 | 0.011 |
Lipoic acid | 5 | mg | 0.005 | 0.024 |
Vitamin B12 | 5 | mg | 0.005 | 0.004 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Defined medium |
Final pH: | 6.5 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 0.988 | 16.909 |
KCl | 0.494 | 6.627 |
MgCl2 x 6 H2O | 0.395 | 1.944 |
NH4Cl | 0.247 | 4.618 |
CaCl2 x 2 H2O | 0.148 | 1.008 |
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