J948
948 .
MODIFIED BALCH'S METHANOGEN MEDIUM 1/B
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J948 #
ml
Equipment needed: Hungate tubes
Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.288 | 2.12 | |
| K2HPO4 | 0.30 | g | 0.288 | 1.656 | |
| NH4Cl | 0.30 | g | 0.288 | 5.393 | |
| NaCl | 0.60 | g | 0.577 | 9.872 | |
| MgCl2 x 6 H2O | 0.13 | g | 0.125 | 0.615 | |
| FeSO4 x 7 H2O | 2.00 | mg | 0.002 | 0.007 | |
| CaCl2 x 2 H2O | 8.00 | mg | 0.008 | 0.052 | |
|
Yeast extract
(BD-Difco) |
0.20 | g | 0.192 | - | |
| Sodium acetate | 2.50 | g | 2.404 | 29.305 | |
| Sodium formate | 2.50 | g | 2.404 | 35.347 | |
| Trace minerals | 10.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| NaHCO3 | 5.00 | g | 4.808 | 57.23 | |
| Resazurin | 1.00 | mg | 9.6e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients, except NaHCO3, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20, v/v), and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., serum bottles, Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand the autoclaved medium overnight. Prior to inoculation, add per liter the following solutions aseptically and anaerobically (autoclaved and stored under a N2 atmosphere): | |||||
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
| 2 Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (80:20, v/v). | |||||
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Hungate tubes |
| Edit in Medium builder | |