Name: NH-JN4 MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J950 #

Equipment needed: Anaerobic workbench Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂SO₄	1.0	g	0.999	7.033
NaCl	30.0	g	29.97	512.834
KCl	0.7	g	0.699	9.38
MgCl₂ x 6 H₂O	5.0	g	4.995	24.569
CaCl₂ x 2 H₂O	1.0	g	0.999	6.795
K₂HPO₄	0.4	g	0.4	2.294
KH₂PO₄	0.4	g	0.4	2.936
NH₄Cl	0.5	g	0.5	9.338
Fe(NH₄)₂(SO₄)₂ x 6 H₂O	2.0	mg	0.002	-
Yeast extract (BD-Difco)	3.0	g	2.997	-
Tryptone (BD-Difco)	4.0	g	3.996	56.218
Sodium acetate	6.8	g	6.793	82.814
L-Cysteine HCl x H₂O	0.6	g	0.599	-
Trace element solution SL-10	1.0	ml	-	-
Resazurin	1.0	mg	1.0e-3	0.004
Distilled water	960.0	ml	-	-
1 Mix components thoroughly and adjust pH to 6.8. Distribute the medium into culture vessels under a N₂-CO₂ (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solution (per 960 ml) from anaerobic stock (filter-sterilized)
NaHCO₃ (5%)	40.0	ml	-	-

Trace element solution SL-10 #

1000

Solution occurs in 20 other media (first defined in Medium J433).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%, 7.7 M)	10.0	ml	2.5	68.568
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
ZnCl₂	70.0	mg	0.07	0.514
MnCl₂ x 4 H₂O	100.0	mg	0.1	0.505
H₃BO₃	6.0	mg	0.006	0.097
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	990.0	ml	-	-
1 Dissolve FeCl₂ x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	6.8
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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