J955
955 .
THERMODESULFOBACTERIUM GEOFONTIS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J955 #
ml
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.000 | g | 2.935 | 20.666 | |
| Na2HPO4 | 0.500 | g | 0.489 | 3.446 | |
| NH4Cl | 1.000 | g | 0.978 | 18.293 | |
| MgCl2 x 6 H2O | 0.400 | g | 0.391 | 1.925 | |
| KCl | 0.500 | g | 0.489 | 6.563 | |
| CaCl2 x 2 H2O | 0.070 | g | 0.068 | 0.466 | |
| FeCl3 x 6 H2O | 2.000 | mg | 0.002 | 0.007 | |
| Sodium acetate | 0.164 | g | 0.16 | 1.956 | |
| HEPES | 2.380 | g | 2.329 | 9.772 | |
| Trace minerals | 10.000 | ml | - | - | |
| Resazurin | 0.500 | mg | 4.9e-4 | 0.002 | |
| Distilled water | 990.000 | ml | - | - | |
| 1 Mix components and adjust pH to 7.0. Bring to a boil for 5-10 sec, then, cool down under a N2-CO2 (80:20, v/v) gas stream. Dispense the medium into culture vessels (e.g., 20 ml/100 ml serum bottle) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions aseptically and anaerobically (autoclaved or *filter-sterilized): | |||||
|
Trace vitamins (see Medium No. 197)
(*) |
10.000 | ml | - | - | |
|
Yeast extract
(10%) |
2.000 | ml | - | - | |
| 2 Just before use, replace the gas phase with a H2-CO2 (80:20, v/v) gas mixture, and add per liter the following solution from sterile anaerobic stock (autoclaved and stored under a N2 atmosphere): | |||||
|
Na2S x 9 H2O
(5%) |
10.000 | ml | - | - | |
| 3 Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (80:20, v/v). | |||||
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |