JCM 957 . DESULFOHALOBIUM UTAHENSE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 100.0 g 99.206 1697.58
MgSO4 x 7 H2O 10.0 g 9.921 40.25
KCl 6.0 g 5.952 79.844
CaCl2 x 2 H2O 0.4 g 0.397 2.699
NH4Cl 1.0 g 0.992 18.547
KH2PO4 0.1 g 0.099 0.729
Yeast extract 0.5 g 0.496 -
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
Resazurin 0.5 mg 5.0e-4 0.002
Distilled water 890.0 ml - -
1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. Aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized) to the medium:
Trace vitamins
(*)
10.0 ml - -
NaHCO3
(*, 8%)
50.0 ml - -
L-Sodium lactate
(5%)
50.0 ml - -
2 Finally, add per liter the following solution from an anaerobic stock:
Na2S x 9 H2O
(5%)
6.0 ml - -
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 99.206 1697.58
MgSO4 x 7 H2O 9.921 40.25
KCl 5.952 79.844
NH4Cl 0.992 18.547
Yeast extract 0.496 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain