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J957. DESULFOHALOBIUM UTAHENSE MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100.0 g 99.206 1697.5
2 10.0 g 9.921 40.249
3 6.0 g 5.952 79.843
4 0.4 g 0.397 2.699
5 1.0 g 0.992 18.546
6 0.1 g 0.099 0.729
7 0.5 g 0.496 -
8 FeCl2 solution 1.0 ml - -
9 Trace element solution 1.0 ml - -
10 0.5 mg 5.0e-4 0.002
11 890.0 ml 881.353 48922.5
12 Trace vitamins
(*)
10.0 ml - -
13
(*, 8%)
50.0 ml 8.567 101.986
14
(5%)
50.0 ml - -
15
(5%)
6.0 ml - -
Instructions:
  1. Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. Aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized) to the medium: #12-14
  2. Finally, add per liter the following solution from an anaerobic stock: #15
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 893.227 49581.6
NaCl 99.206 1697.5
MgSO4 x 7H2O 9.921 40.249
NaHCO3 8.567 101.986
KCl 5.952 79.843
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