MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 957 . DESULFOHALOBIUM UTAHENSE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J957 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 100.0 g 99.206 1697.58
MgSO4 x 7 H2O 10.0 g 9.921 40.25
KCl 6.0 g 5.952 79.844
CaCl2 x 2 H2O 0.4 g 0.397 2.699
NH4Cl 1.0 g 0.992 18.547
KH2PO4 0.1 g 0.099 0.729
Yeast extract 0.5 g 0.496 -
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
Resazurin 0.5 mg 5.0e-4 0.002
Distilled water 890.0 ml - -
1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. Aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized) to the medium:
Trace vitamins
(*) 		10.0 ml - -
NaHCO3
(*, 8%) 		50.0 ml - -
L-Sodium lactate
(5%) 		50.0 ml - -
2 Finally, add per liter the following solution from an anaerobic stock:
Na2S x 9 H2O
(5%) 		6.0 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 99.206 1697.58
MgSO4 x 7 H2O 9.921 40.25
KCl 5.952 79.844
NH4Cl 0.992 18.547
Yeast extract 0.496 -
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain