J959
959 .
THERMANAEROBACTER THERMOPHILA MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J959 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.297 | 2.185 | |
| K2HPO4 | 0.30 | g | 0.297 | 1.707 | |
| NH4Cl | 0.30 | g | 0.297 | 5.559 | |
| NaCl | 1.00 | g | 0.991 | 16.959 | |
| MgCl2 x 6 H2O | 0.50 | g | 0.496 | 2.437 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.149 | 1.011 | |
| KCl | 0.10 | g | 0.099 | 1.329 | |
|
Trace element solution
(see Medium No. 294) |
1.00 | ml | - | - | |
| Yeast extract | 0.50 | g | 0.496 | - | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 935.00 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.2 with KOH, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 935 ml of the medium) from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
25.00 | ml | - | - | |
|
Glucose
(1 M) |
20.00 | ml | - | - | |
|
Na2S2O3 x 5 H2O
(*, 1 M) |
20.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 0.991 | 16.959 |
| MgCl2 x 6 H2O | 0.496 | 2.437 |
| Yeast extract | 0.496 | - |
| NH4Cl | 0.297 | 5.559 |
| KH2PO4 | 0.297 | 2.185 |
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