Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Sea Salt
(Sigma) |
30.00 | g | 28.846 | - | |
KH2PO4 | 0.14 | g | 0.135 | 0.989 | |
NH4Cl | 0.25 | g | 0.24 | 4.494 | |
Sodium pyruvate | 1.10 | g | 1.058 | 9.612 | |
Resazurin | 1.00 | mg | 9.6e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components thoroughly, bring to a boil and cool down to room temperature under a N2-CO2 (4:1, v/v) gas stream. Dispense the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
Trace vitamins
(*) |
10.00 | ml | - | - | |
NaHCO3
(*, 8%) |
20.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
2 Check final pH to be 7.0 - 7.2. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 03.04.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.1 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Sea Salt | 28.846 | - |
Sodium pyruvate | 1.058 | 9.612 |
NH4Cl | 0.24 | 4.494 |
KH2PO4 | 0.135 | 0.989 |
Resazurin | 9.6e-4 | 0.004 |
Show more |