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JCM 971 . 1/2 SME MEDIUM FOR YA

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J971 #

ml
Equipment needed: Autoclave Anaerobic workbench
1 Mix components thoroughly, boil for a few seconds and cool down under a stream of a N2-CO2(4:1, v/v) gas mixture. After cooling, add the following components and adjust pH to 7.0:
Compound Amount Unit Conc. [g/L] Conc. [mM]
Synthetic seawater (2 x)
(2 x) 		250.000 ml - -
KH2PO4 0.500 g 0.452 3.319
NH4Cl 0.300 g 0.271 5.066
Sodium acetate trihydrate 1.080 g 0.976 7.169
Yeast extract 0.200 g 0.181 -
Modified Wolfe's minerals
(10 x, see below) 		1.000 ml - -
Trace vitamins 10.000 ml - -
Resazurin 1.000 mg 9.0e-4 0.004
Distilled water 740.000 ml - -
NaHCO3 3.000 g 2.71 32.26
Na2S x 9 H2O
(5%) 		6.000 ml - -
2 Modified Wolfe's minerals (10 x):
MgSO4 x 7 H2O 3.000 g 2.71 10.995
MnSO4 x n H2O 0.500 g 0.452 -
NaCl 1.000 g 0.903 15.458
FeSO4 x 7 H2O 0.100 g 0.09 0.325
CoSO4 x 7 H2O 0.180 g 0.163 1.049
CaCl2 x 2 H2O 0.100 g 0.09 0.614
ZnSO4 x 7 H2O 0.180 g 0.163 0.565
CuSO4 x 5 H2O 0.010 g 0.009 0.036
AlK(SO4)2 x 12 H2O 0.018 g 0.016 0.034
H3BO3 0.010 g 0.009 0.146
Na2MoO4 x 2 H2O 0.010 g 0.009 0.037
(NH4)2Ni(SO4)2 x 6 H2O 0.280 g 0.253 0.64
Na2SeO4 0.010 g 0.009 0.048
Na2WO4 x 2 H2O 0.010 g 0.009 0.027
Distilled water 100.000 ml - -
3 Dispense the medium into culture vessels anaerobically under the same gas mixture, seal with butyl rubber stoppers and autoclave.
4 Adjust pH to 1.0 with 1 N H2SO4.

Synthetic seawater (2 x) #

1000
Solution occurs in 1 other media (first defined in Medium J590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 55.40 g 55.4 947.981
MgSO4 x 7 H2O 14.00 g 14 56.801
MgCl2 x 6 H2O 11.00 g 11 54.106
KCl 1.30 g 1.3 17.438
NaBr 0.20 g 0.2 1.944
H3BO3 0.06 g 0.06 0.97
SrCl2 x 6 H2O 0.03 g 0.03 0.113
KI 0.10 mg 1.0e-4 3.9e-4
CaCl2 x 2 H2O 1.50 g 1.5 10.203
Distilled water 1000.00 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 1.0
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 13.415 229.545
MgSO4 x 7 H2O 5.872 23.823
NaHCO3 2.71 32.26
MgCl2 x 6 H2O 2.484 12.219
Sodium acetate trihydrate 0.976 7.169
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