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J974. ALKALINUS ABYSSINIESE MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 117.0 g 1017.39 17408.4
2
(BD-Difco)
5.0 g 43.478 -
3
(Oxoid)
5.0 g 43.478 -
4 0.2 g 1.739 7.056
5 0.2 g 1.739 11.83
6 0.1 g 0.87 6.39
7 Trace mineral solution 10.0 ml - -
8
(w/v, 10%)
5.0 ml - -
9
(w/v, 10%)
100.0 ml 220 2075.71
Instructions:
  1. Add components to distilled water and bring volume to 900 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add aseptically the following solutions (separately autoclaved): #8-9
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30 mg 0.03 0.385
2 10 mg 0.01 -
3 3 mg 0.003 0.012
4 3 mg 0.003 -
5 2 mg 0.002 -
6 10 mg 0.01 -
7 1 mg 1.0e-3 -
8 1000 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 1017.39 17408.4
Na2CO3 220 2075.71
Distilled water 86.801 4818.16
Yeast extract 43.478 -
Peptone 43.478 -
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