J974
974 .
ALKALINUS ABYSSINIESE MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J974 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 117.0 | g | 1017.39 | 17409.2 | |
|
Yeast extract
(BD-Difco) |
5.0 | g | 43.478 | - | |
|
Peptone
(Oxoid) |
5.0 | g | 43.478 | - | |
| MgSO4 x 7 H2O | 0.2 | g | 1.739 | 7.056 | |
| CaCl2 x 2 H2O | 0.2 | g | 1.739 | 11.83 | |
| KH2PO4 | 0.1 | g | 0.87 | 6.39 | |
| Trace mineral solution | 10.0 | ml | - | - | |
| 1 Add components to distilled water and bring volume to 900 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add aseptically the following solutions (separately autoclaved): | |||||
|
Glucose
(w/v, 10%) |
5.0 | ml | - | - | |
|
Na2CO3
(w/v, 10%) |
100.0 | ml | - | - | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| H3BO4 | 30 | mg | 0.03 | - |
| ZnSO4 x 7 H2O | 10 | mg | 0.01 | 0.035 |
| Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 |
| MnCl2 x 4 H2O | 3 | mg | 0.003 | - |
| NiCl2 x 6 H2O | 2 | mg | 0.002 | 0.015 |
| CoCl2 x 6 H2O | 10 | mg | 0.01 | 0.077 |
| CuCl2 x 2 H2O | 1 | mg | 1.0e-3 | 0.006 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |