J975
975 .
SALINIVIBRIO SHARMENSIS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J975 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 100.00 | g | 100 | 1711.16 | |
| Trisodium citrate | 3.00 | g | 3 | 11.625 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| KCl | 2.00 | g | 2 | 26.828 | |
| MnCl2 x 4 H2O | 0.36 | mg | 3.6e-4 | 0.002 | |
| FeSO4 x 7 H2O | 0.05 | g | 0.05 | 0.18 | |
|
Yeast extract
(BD-Difco) |
10.00 | g | 10 | - | |
| 1 Add components to distilled water and bring volume to 970 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add the following solution (separately autoclaved): | |||||
|
Na2CO3
(w/v, 10%) |
30.00 | ml | 3 | 28.305 | |
| 2 Check the final pH to be around 9.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 100 | 1711.16 |
| Yeast extract | 10 | - |
| Na2CO3 | 3 | 28.305 |
| Trisodium citrate | 3 | 11.625 |
| KCl | 2 | 26.828 |
| Show more | ||
