Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
1 Mix components thoroughly and adjust pH to 6.75 with NaOH. Distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (filter-sterilized and stored anaerobically): | |||||
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 3.000 | g | 2.927 | 50.083 | |
KCl | 0.150 | g | 0.146 | 1.963 | |
Na2SO4 | 0.300 | g | 0.293 | 2.061 | |
MgSO4 x 7 H2O | 0.123 | g | 0.12 | 0.487 | |
CaCl2 x 2 H2O | 0.015 | g | 0.015 | 0.1 | |
NH4Cl | 0.107 | g | 0.104 | 1.952 | |
NaH2PO4 | 1.380 | g | 1.346 | 11.222 | |
Mineral solution | 5.000 | ml | - | - | |
Distilled water | 1000.000 | ml | - | - | |
Trace vitamins | 10.000 | ml | - | - | |
Peptone
(w/v, 10%) |
10.000 | ml | - | - | |
2 Before inoculation, add filter-sterilized O2 gas or air, at a final concentration of 1% O2 in the gas phase. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
EDTA | 3.7 | g | 3.7 | 12.661 | |
FeSO4 x 7 H2O | 1.1 | g | 1.1 | 3.957 | |
MnCl2 x 4 H2O | 98.4 | mg | 0.098 | - | |
ZnSO4 x 7 H2O | 28.8 | mg | 0.029 | 0.1 | |
CoCl2 x 6 H2O | 23.8 | mg | 0.024 | 0.183 | |
CuCl2 x 2 H2O | 17.2 | mg | 0.017 | 0.101 | |
Na2MoO4 x 2 H2O | 24.2 | mg | 0.024 | 0.1 | |
H3BO3 | 31.0 | mg | 0.031 | 0.501 | |
Distilled water | 1000.0 | ml | - | - | |
1 Adjust pH to 6.0 with KOH. | |||||
2 Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.0 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 2.927 | 50.083 |
NaH2PO4 | 1.346 | 11.222 |
Na2SO4 | 0.293 | 2.061 |
KCl | 0.146 | 1.963 |
MgSO4 x 7 H2O | 0.12 | 0.487 |
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