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J977. VNG MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 x CMD stock solution 100.000 ml - -
2 Trace vitamins 10.000 ml - -
3 0.027 g 0.027 0.505
4 3.570 g 3.57 -
5 790.000 ml 788.584 43773
6
(w/v, 20%)
100.000 ml - -
Instructions:
  1. Mix components thoroughly, adjust pH to 8.1 with NaOH or HCl. Distribute the medium into culture vessels (e.g., 9 ml in 25 ml serum bottles/Balch tubes), seal with butyl rubber stoppers and autoclave. After cooling, add the following solution (per 900 ml, autoclaved): #6
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.999 5.227
2 5 x CMD micronutrient solution 1.00 ml - -
3 4.80 mg 0.005 -
4 0.60 g 0.599 -
5 1.00 g 0.999 4.053
6 0.80 g 0.799 13.675
7 1.03 g 1.029 10.178
8 6.89 g 6.883 80.983
9 1.11 g 1.109 9.243
10 1000.00 ml 997.21 55353.5
Instructions:
  1. Do not use if exceeds 90 days shelf life.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100.00 ml 998.207 55408.8
2
(6.25 N)
0.25 ml - -
3 1.14 g 11.4 -
4 0.25 g 2.5 -
5 0.25 g 2.5 40.432
6 8.50 mg 0.085 -
7 12.50 mg 0.125 0.517
8 22.50 mg 0.225 -
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 898.387 49868
HEPES 3.57 -
NaNO3 0.688 8.098
NaH2PO4 0.111 0.924
KNO3 0.103 1.018
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