J977
977 .
VNG MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J977 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 10 x CMD stock solution | 100.000 | ml | - | - | |
| Trace vitamins | 10.000 | ml | - | - | |
| NH4Cl | 0.027 | g | 0.027 | 0.505 | |
| HEPES | 3.570 | g | 3.57 | 14.981 | |
| Distilled water | 790.000 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 8.1 with NaOH or HCl. Distribute the medium into culture vessels (e.g., 9 ml in 25 ml serum bottles/Balch tubes), seal with butyl rubber stoppers and autoclave. After cooling, add the following solution (per 900 ml, autoclaved): | |||||
|
Glucose
(w/v, 20%) |
100.000 | ml | - | - | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.00 | g | 0.999 | 5.227 | |
| 5 x CMD micronutrient solution | 1.00 | ml | - | - | |
| FeCl3 x 6 H2O | 4.80 | mg | 0.005 | 0.018 | |
| CaSO4 x 2 H2O | 0.60 | g | 0.599 | 3.481 | |
| MgSO4 x 7 H2O | 1.00 | g | 0.999 | 4.053 | |
| NaCl | 0.80 | g | 0.799 | 13.676 | |
| KNO3 | 1.03 | g | 1.029 | 10.178 | |
| NaNO3 | 6.89 | g | 6.883 | 80.983 | |
| NaH2PO4 | 1.11 | g | 1.109 | 9.243 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Do not use if exceeds 90 days shelf life. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Distilled water | 100.00 | ml | - | - |
|
H2SO4
(6.25 N) |
0.25 | ml | - | - |
| MnSO4 x n H2O | 1.14 | g | 11.4 | - |
| ZnSO4 x 7 H2O | 0.25 | g | 2.5 | 8.694 |
| H3BO3 | 0.25 | g | 2.5 | 40.427 |
| CuCl2 x 2 H2O | 8.50 | mg | 0.085 | 0.499 |
| Na2MoO4 x 2 H2O | 12.50 | mg | 0.125 | 0.517 |
| CoCl2 x 6 H2O | 22.50 | mg | 0.225 | 1.733 |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 8.1 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| HEPES | 3.57 | 14.981 |
| NaNO3 | 0.688 | 8.098 |
| NaH2PO4 | 0.111 | 0.924 |
| KNO3 | 0.103 | 1.018 |
| Nitrilotriacetic acid | 0.1 | 0.523 |
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