J98
98 .
ALKALINE LACTOSE AGAR
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J98 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Lactose | 5.0 | g | 5 | 14.607 | |
| Yeast extract | 5.0 | g | 5 | - | |
|
Polypeptone
(Nihon Pharm. Co.) |
5.0 | g | 5 | - | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Distilled water | 900.0 | ml | - | - | |
|
Na2CO3
(10%) |
100.0 | ml | - | - | |
| 1 Separately sterilize Na2CO3 solution by filtration, and aseptically add to the medium. Check final pH to be about 10.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 10.0 |
| Equipment needed: | Filter |
| Edit in Medium builder | |