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J983. ARTIFICIAL SEAWATER MEDIUM WITH PULLULAN

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 80.0 g 75.901 1298.74
2 25.0 g 23.719 96.232
3 4.5 g 4.269 29.042
4 1.5 g 1.423 19.09
5 FeCl2 solution 1.0 ml - -
6 Trace element solution 1.0 ml - -
7 Selenite-tungstate solution 1.0 ml - -
8 0.5 mg 4.7e-4 0.002
9 0.5 g 0.474 -
10 1000.0 ml 947.065 52570.1
11
(1%)
10.0 ml 1.5e-4 0.003
12
(1%)
5.0 ml 0.218 1.604
13 Trace vitamins
(*)
10.0 ml - -
14
(5%)
20.0 ml 2.4 22.648
15
(5%)
6.0 ml - -
Instructions:
  1. Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized and stored under N2): #11-15
  2. Adjust pH to 7.2 - 7.5 with a 5% Na2CO3 solution, if necessary.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 959.367 53253
NaCl 75.901 1298.74
MgSO4 x 7H2O 23.719 96.232
CaCl2 x 2H2O 4.269 29.042
Na2CO3 2.4 22.648
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