983 .
ARTIFICIAL SEAWATER MEDIUM WITH PULLULAN
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 80.0 | g | ||
|
|
MgSO4 x 7 H2O | 25.0 | g | ||
|
|
CaCl2 x 2 H2O | 4.5 | g | ||
|
|
KCl | 1.5 | g | ||
|
|
FeCl2 solution | 1.0 | ml | ||
|
|
Trace element solution | 1.0 | ml | ||
|
|
Selenite-tungstate solution | 1.0 | ml | ||
|
|
Resazurin | 0.5 | mg | ||
|
|
Pullulan | 0.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized and stored under N2): | |||||
|
|
NH4Cl
(1%) |
10.0 | ml | ||
|
|
KH2PO4
(1%) |
5.0 | ml | ||
|
|
Trace vitamins
(*) |
10.0 | ml | ||
|
|
Na2CO3
(5%) |
20.0 | ml | ||
|
|
Na2S x 9 H2O
(5%) |
6.0 | ml | ||
| 2 Adjust pH to 7.2 - 7.5 with a 5% Na2CO3 solution, if necessary. | |||||
FeCl2 solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
Distilled water | 990.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.4 | g |
|
|
Na2SeO3 x 5 H2O | 6.0 | mg |
|
|
Na2WO4 x 2 H2O | 8.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |