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JCM 985 . METHANOMETHYLOVORANS UPONENSIS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J985 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.0 g 0.989 16.925
KCl 0.5 g 0.495 6.634
MgCl2 x 6 H2O 0.4 g 0.396 1.946
CaCl2 x 2 H2O 0.1 g 0.099 0.673
KH2PO4 0.2 g 0.198 1.454
NH4Cl 0.5 g 0.495 9.246
Resazurin 1.0 mg 9.9e-4 0.004
Distilled water 960.0 ml - -
1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
Trace vitamins
(*) 		10.0 ml - -
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Trimethylamine-HCl
(*, 30%) 		10.0 ml - -
NaHCO3
(*, 8%) 		20.0 ml - -
2 Aseptically and anaerobically distribute the medium into culture vessels under a N2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
Na2S x 9 H2O
(5%) 		8.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.989 16.925
NH4Cl 0.495 9.246
KCl 0.495 6.634
MgCl2 x 6 H2O 0.396 1.946
KH2PO4 0.198 1.454
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain