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JCM 987 . CEJFB1T MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.991 18.528
K2HPO4 0.5 g 0.496 2.845
CaCl2 x 2 H2O 0.1 g 0.099 0.674
KCl 0.8 g 0.793 10.635
Sodium acetate 0.5 g 0.496 6.041
NaCl 200.0 g 198.216 3391.79
Yeast extract
(BD-Difco)
1.0 g 0.991 -
Tryptone
(BD-Difco)
0.5 g 0.496 6.972
Trace element solution 1.0 ml - -
L-Cysteine HCl x H2O 0.5 g 0.496 -
Resazurin 0.5 mg 5.0e-4 0.002
Distilled water 855.0 ml - -
1 Mix components thoroughly, adjust pH to 7.6 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%)
25.0 ml - -
Glucose
(1 M)
20.0 ml - -
MgSO4 x 7 H2O
(10%)
50.0 ml - -
Tryptone
(10%)
50.0 ml - -
2 Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere).
Na2S x 9 H2O
(5%)
8.0 ml - -
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.6
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 198.216 3391.79
NH4Cl 0.991 18.528
Yeast extract 0.991 -
KCl 0.793 10.635
Tryptone 0.496 6.972
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain
temperature thermophilic 1 strain

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