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JCM 989 . IRD AMINOBACTERIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.998 18.658
KH2PO4 0.3 g 0.299 2.2
K2HPO4 0.3 g 0.299 1.719
NaCl 20.0 g 19.96 341.548
KCl 0.1 g 0.1 1.339
CaCl2 x 2 H2O 0.1 g 0.1 0.679
L-Serine 1.0 g 0.998 9.497
Yeast extract
(BD-Difco)
2.0 g 1.996 -
Trace element solution 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
L-Cysteine HCl x H2O 0.5 g 0.499 -
Resazurin 0.5 mg 5.0e-4 0.002
Distilled water 945.0 ml - -
1 Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N2 atmosphere or *filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture]:
MgCl2 x 6 H2O
(15%)
20.0 ml - -
NaHCO3
(*, 8%)
25.0 ml - -
Na2S x 9 H2O
(5%)
10.0 ml - -
2 Readjust pH to 7.2, if necessary.
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.96 341.548
Yeast extract 1.996 -
NH4Cl 0.998 18.658
L-Serine 0.998 9.497
L-Cysteine HCl x H2O 0.499 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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