Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.0 | g | 0.998 | 18.658 | |
KH2PO4 | 0.3 | g | 0.299 | 2.2 | |
K2HPO4 | 0.3 | g | 0.299 | 1.719 | |
NaCl | 20.0 | g | 19.96 | 341.548 | |
KCl | 0.1 | g | 0.1 | 1.339 | |
CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.679 | |
L-Serine | 1.0 | g | 0.998 | 9.497 | |
Yeast extract
(BD-Difco) |
2.0 | g | 1.996 | - | |
Trace element solution | 1.0 | ml | - | - | |
Selenite-tungstate solution | 1.0 | ml | - | - | |
L-Cysteine HCl x H2O | 0.5 | g | 0.499 | - | |
Resazurin | 0.5 | mg | 5.0e-4 | 0.002 | |
Distilled water | 945.0 | ml | - | - | |
1 Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N2 atmosphere or *filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture]: | |||||
MgCl2 x 6 H2O
(15%) |
20.0 | ml | - | - | |
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
2 Readjust pH to 7.2, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(25%) |
12.5 | ml | - | - |
FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
H3BO3 | 30.0 | mg | 0.03 | 0.485 |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
Distilled water | 987.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 19.96 | 341.548 |
Yeast extract | 1.996 | - |
NH4Cl | 0.998 | 18.658 |
L-Serine | 0.998 | 9.497 |
L-Cysteine HCl x H2O | 0.499 | - |
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