J994
994 .
MBM MEDIUM 2
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J994 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 80.0 | g | 78.278 | 1339.46 | |
| MgSO4 x 7 H2O | 6.0 | g | 5.871 | 23.819 | |
| KCl | 1.5 | g | 1.468 | 19.688 | |
| NH4Cl | 1.0 | g | 0.978 | 18.293 | |
| CaCl2 x 2 H2O | 0.4 | g | 0.391 | 2.662 | |
| K2HPO4 | 0.4 | g | 0.391 | 2.247 | |
| Trace minerals | 10.0 | ml | - | - | |
| Sodium acetate x 3 H2O | 1.6 | g | 1.566 | - | |
| Resazurin | 1.0 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 900.0 | ml | - | - | |
| 1 Mix components thoroughly. Bring to a boil for a few seconds, cool down under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
|
Disodium fumarate
(1 M) |
40.0 | ml | - | - | |
|
NaHCO3
(*, 8%) |
50.0 | ml | - | - | |
|
Trace vitamins
(*) |
10.0 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 gas atmosphere): | |||||
|
L-Cysteine HCl x H2O
(5%) |
6.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
6.0 | ml | - | - | |
| 3 Readjust pH to 7.5, if necessary. | |||||
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 7.5 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
| Edit in Medium builder | |