Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Modified Hutner's basal salts | 20 | ml | - | - | |
Sea water
(aged) |
700 | ml | - | - | |
Tris-HCl buffer
(pH 7.5, 0.1 M) |
50 | ml | - | - | |
Distilled water | 260 | ml | - | - | |
1 For solid medium, add 5.0 g/L gelrite (Gellan Gum). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
N-Acetyl-D-glucosamine
(Sigma) |
2.00 | g | 37.037 | 167.429 | |
Na2HPO4 x 2 H2O | 0.01 | g | 0.185 | 1.305 | |
Ampicillin
(50 mg/ml) |
4.00 | ml | - | - | |
Vitamin solution No. 6 | 10.00 | ml | - | - | |
Distilled water | 40.00 | ml | - | - | |
1 Add 0.2 ml filter-sterilized Solution 2 to Solution 1. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Biotin | 4.0 | mg | 0.004 | 0.016 | |
Pyridoxine hydrochloride | 20.0 | mg | 0.02 | - | |
Thiamine-HCl x 2 H2O | 10.0 | mg | 0.01 | - | |
Calcium pantothenate | 10.0 | mg | 0.01 | 0.021 | |
p-Aminobenzoic acid | 10.0 | mg | 0.01 | 0.073 | |
Folic acid | 4.0 | mg | 0.004 | 0.009 | |
Riboflavin | 10.0 | mg | 0.01 | 0.027 | |
Nicotinamide | 10.0 | mg | 0.01 | 0.082 | |
Vitamin B12 | 0.2 | mg | 2.0e-4 | 1.5e-4 | |
Distilled water | 1000.0 | ml | - | - | |
1 Filter-sterilize. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | - | |
FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
Metals ''44'' | 50.000 | ml | - | - | |
Distilled water | 950.000 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4. |
Last modified: | 03.04.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Filter |
Edit in Medium builder |
All strains for this medium: | 1 |